Jones BS, Pareek V, Hu DD, Weaver SD, Syska C, Galfano G, Champion MM, Champion PA. N-acetyltransferases required for iron uptake and aminoglycoside resistance promote virulence lipid production in Mycobacterium marinum. Proc Natl Acad Sci U S A. 2025 Jul 22;122(29):e2502577122. doi: 10.1073/pnas.2502577122. Epub 2025 Jul 18. PMID: 40680026; PMCID: PMC12305045.
Reynolds TS, Hu D, Weaver SD, Ronck EC, Mishra SJ, Champion MM, Blagg BSJ. Proteomic Analysis of Hsp90β-selective Inhibitors Against Triple-negative Breast Cancer (TNBC) to Gain a Mechanistic Insight. Mol Cell Proteomics. 2025 Jul 24:101043. doi: 10.1016/j.mcpro.2025.101043. Epub ahead of print. PMID: 40714319.
Kerr CM, Schneider OL, Tichy S, Huge BJ, Champion MM. Capillary Isoelectric Focusing of Proteins and Peptides Using an In-Line cIEF-ESI Interface with Improved MS Characteristics. Anal Chem. 2025 Jan 1. doi: 10.1021/acs.analchem.4c05010. Epub ahead of print. PMID: 39742429.
Rodriguez A, Diehl JD, Wright GS, Bonar CD, Lundgren TJ, Moss MJ, Li J, Milenkovic T, Huber PW, Champion MM, Emrich SJ, Clark PL. Synonymous codon substitutions modulate transcription and translation of a divergent upstream gene by modulating antisense RNA production. Proc Natl Acad Sci U S A. 2024 Sep 3;121(36):e2405510121. doi: 10.1073/pnas.2405510121. Epub 2024 Aug 27. PMID: 39190361; PMCID: PMC11388325.
Mousseau CB, Hu DD, Schultz SR, Champion MM. Quenching Trypsin Is Unnecessary in Filter-Based Bottom-Up Proteomics. J Am Soc Mass Spectrom. 2024 Jul 9. doi: 10.1021/jasms.4c00143. Epub ahead of print. PMID: 38982799.
Jones BS, Hu DD, Nicholson KR, Cronin RM, Weaver SD, Champion MM, Champion PA. The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 substrates in Mycobacterium marinum. mSphere. 2024 Apr 25:e0000524. doi: 10.1128/msphere.00005-24. PMID: 38661343.
Atomwise AIMS Program. AI is a viable alternative to high throughput screening: a 318-target study. Sci Rep. 2024 Apr 2;14(1):7526. doi: 10.1038/s41598-024-54655-z. PMID: 38565852; PMCID: PMC10987645.
Lundgren TJ, Clark PL, Champion MM. Fit for Purpose Approach To Evaluate Detection of Amino Acid Substitutions in Shotgun Proteomics. J Proteome Res. 2024 Mar 13. doi: 10.1021/acs.jproteome.3c00730. PMID: 38478054.
Hu DD, Weaver SD, Collars OA, Champion PA, Champion MM. N-terminal proteomics of Mycobacterium marinum using bottom-up label-free quantitative analysis in data-dependent acquisition mode on a timsTOF Pro mass spectrometer. Microbiol Resour Announc. 2024 Mar 13:e0126323. doi: 10.1128/mra.01263-23. Epub ahead of print. PMID: 38477461.
Huge, B.J., Kerr, C.M., Wanigasinghe, S. Champion, M.M., & Dovichi, N.J. Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B. Sci Rep 13, 22269 (2023). https://doi.org/10.1038/s41598-023-49669-y
Collars, O. A., Jones, B. S., Hu, D. D., Weaver, S. D., Sherman, T. A., Champion, M. M., & Champion, P. A. (2023). An N-acetyltransferase required for ESAT-6 N-terminal acetylation and virulence in Mycobacterium marinum. MBio. https://doi.org/10.1128/mbio.00987-23
Weaver SD, DeRosa CM, Schultz SR, Champion MM. PrIntMap-R: An Online Application for Intraprotein Intensity and Peptide Visualization from Bottom-Up Proteomics. J Proteome Res. 2023 Feb 3;22(2):432-441. doi: 10.1021/acs.jproteome.2c00606. Epub 2023 Jan 18. PMID: 36652611.
Mousseau CB, Pierre CA, Hu DD, Champion MM. Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation. Anal Methods. 2023 Feb 16;15(7):916-924. doi: 10.1039/d2ay01549h. PMID: 36373982.
Saelens JW, Sweeney MI, Viswanathan G, Xet-Mull AM, Jurcic Smith KL, Sisk DM, Hu DD, Cronin RM, Hughes EJ, Brewer WJ, Coers J, Champion MM, Champion PA, Lowe CB, Smith CM, Lee S, Stout JE, Tobin DM. An ancestral mycobacterial effector promotes dissemination of infection. Cell. 2022 Nov 23;185(24):4507-4525.e18. doi: 10.1016/j.cell.2022.10.019. Epub 2022 Nov 9. PMID: 36356582; PMCID: PMC9691622.
Cronin RM, Ferrell MJ, Cahir CW, Champion MM, Champion PA. Proteo-genetic analysis reveals clear hierarchy of ESX-1 secretion in Mycobacterium marinum. Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2123100119. doi: 10.1073/pnas.2123100119. Epub 2022 Jun 7. PMID: 35671426; PMCID: PMC9214503.
Smith C, Canestrari JG, Wang AJ, Champion MM, Derbyshire KM, Gray TA, Wade JT. Pervasive translation in Mycobacterium tuberculosis. Elife. 2022 Mar 28;11:e73980. doi: 10.7554/eLife.73980. PMID: 35343439; PMCID: PMC9094748.
Huge BJ, Young K, Kerr C, Champion MM, Dovichi NJ. 3-D printed injection system for capillary electrophoresis. Anal Methods. 2022 Mar 17;14(11):1163-1168. doi: 10.1039/d2ay00075j. PMID: 35254370; PMCID: PMC8934206.
Ahrens CH, Wade JT, Champion MM, Langer JD. A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry. J Bacteriol. 2022 Jan 18;204(1):e0035321. doi: 10.1128/JB.00353-21. Epub 2021 Nov 8. PMID: 34748388; PMCID: PMC8765459.
Nicholson KR, Mousseau CB, Champion MM, Champion PA. The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens. PLoS Pathog. 2021 Jan 7;17(1):e1009124. doi: 10.1371/journal.ppat.1009124. PMID: 33411813; PMCID: PMC7790235.
Yan X, Sun L, Dovichi NJ, Champion MM. Minimal deuterium isotope effects in quantitation of dimethyl-labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry. Electrophoresis. 2020 Aug;41(15):1374-1378. doi: 10.1002/elps.202000051. Epub 2020 Jun 16. PMID: 32548848; PMCID: PMC7540333.
Canestrari JG, Lasek-Nesselquist E, Upadhyay A, Rofaeil M, Champion MM, Wade JT, Derbyshire KM, Gray TA. Polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene expression in mycobacteria. Mol Microbiol. 2020 Jul;114(1):93-108. doi: 10.1111/mmi.14498. Epub 2020 Apr 9. PMID: 32181921; PMCID: PMC8764745.
Sanchez KG, Ferrell MJ, Chirakos AE, Nicholson KR, Abramovitch RB, Champion MM, Champion PA. EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System. mBio. 2020 Feb 4;11(1):e02807-19. doi: 10.1128/mBio.02807-19. PMID: 32019792; PMCID: PMC7002343.
Bosserman RE, Nicholson KR, Champion MM, Champion PA. A New ESX-1 Substrate in Mycobacterium marinum That Is Required for Hemolysis but Not Host Cell Lysis. J Bacteriol. 2019 Jun 21;201(14):e00760-18. doi: 10.1128/JB.00760-18. PMID: 30833360; PMCID: PMC6597391.
Huge BJ, Champion MM, Dovichi NJ. Capillary Zone Electrophoresis with Fraction Collection for Separation, Culturing, and Identification of Bacteria from an Environmental Microbiome. Anal Chem. 2019 Apr 2;91(7):4649-4655. doi: 10.1021/acs.analchem.8b05984. Epub 2019 Mar 11. PMID: 30801180.
Thompson CR, Champion MM, Champion PA. Quantitative N-Terminal Footprinting of Pathogenic Mycobacteria Reveals Differential Protein Acetylation. J Proteome Res. 2018 Sep 7;17(9):3246-3258. doi: 10.1021/acs.jproteome.8b00373. Epub 2018 Aug 16. PMID: 30080413; PMCID: PMC6264890.
Reynolds TS, Hu D, Weaver SD, Ronck EC, Mishra SJ, Champion MM, Blagg BSJ. Proteomic Analysis of Hsp90β-selective Inhibitors Against Triple-negative Breast Cancer (TNBC) to Gain a Mechanistic Insight. Mol Cell Proteomics. 2025 Jul 24:101043. doi: 10.1016/j.mcpro.2025.101043. Epub ahead of print. PMID: 40714319.
Kerr CM, Schneider OL, Tichy S, Huge BJ, Champion MM. Capillary Isoelectric Focusing of Proteins and Peptides Using an In-Line cIEF-ESI Interface with Improved MS Characteristics. Anal Chem. 2025 Jan 1. doi: 10.1021/acs.analchem.4c05010. Epub ahead of print. PMID: 39742429.
Rodriguez A, Diehl JD, Wright GS, Bonar CD, Lundgren TJ, Moss MJ, Li J, Milenkovic T, Huber PW, Champion MM, Emrich SJ, Clark PL. Synonymous codon substitutions modulate transcription and translation of a divergent upstream gene by modulating antisense RNA production. Proc Natl Acad Sci U S A. 2024 Sep 3;121(36):e2405510121. doi: 10.1073/pnas.2405510121. Epub 2024 Aug 27. PMID: 39190361; PMCID: PMC11388325.
Mousseau CB, Hu DD, Schultz SR, Champion MM. Quenching Trypsin Is Unnecessary in Filter-Based Bottom-Up Proteomics. J Am Soc Mass Spectrom. 2024 Jul 9. doi: 10.1021/jasms.4c00143. Epub ahead of print. PMID: 38982799.
Jones BS, Hu DD, Nicholson KR, Cronin RM, Weaver SD, Champion MM, Champion PA. The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 substrates in Mycobacterium marinum. mSphere. 2024 Apr 25:e0000524. doi: 10.1128/msphere.00005-24. PMID: 38661343.
Atomwise AIMS Program. AI is a viable alternative to high throughput screening: a 318-target study. Sci Rep. 2024 Apr 2;14(1):7526. doi: 10.1038/s41598-024-54655-z. PMID: 38565852; PMCID: PMC10987645.
Lundgren TJ, Clark PL, Champion MM. Fit for Purpose Approach To Evaluate Detection of Amino Acid Substitutions in Shotgun Proteomics. J Proteome Res. 2024 Mar 13. doi: 10.1021/acs.jproteome.3c00730. PMID: 38478054.
Hu DD, Weaver SD, Collars OA, Champion PA, Champion MM. N-terminal proteomics of Mycobacterium marinum using bottom-up label-free quantitative analysis in data-dependent acquisition mode on a timsTOF Pro mass spectrometer. Microbiol Resour Announc. 2024 Mar 13:e0126323. doi: 10.1128/mra.01263-23. Epub ahead of print. PMID: 38477461.
Huge, B.J., Kerr, C.M., Wanigasinghe, S. Champion, M.M., & Dovichi, N.J. Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B. Sci Rep 13, 22269 (2023). https://doi.org/10.1038/s41598-023-49669-y
Collars, O. A., Jones, B. S., Hu, D. D., Weaver, S. D., Sherman, T. A., Champion, M. M., & Champion, P. A. (2023). An N-acetyltransferase required for ESAT-6 N-terminal acetylation and virulence in Mycobacterium marinum. MBio. https://doi.org/10.1128/mbio.00987-23
Weaver SD, DeRosa CM, Schultz SR, Champion MM. PrIntMap-R: An Online Application for Intraprotein Intensity and Peptide Visualization from Bottom-Up Proteomics. J Proteome Res. 2023 Feb 3;22(2):432-441. doi: 10.1021/acs.jproteome.2c00606. Epub 2023 Jan 18. PMID: 36652611.
Mousseau CB, Pierre CA, Hu DD, Champion MM. Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation. Anal Methods. 2023 Feb 16;15(7):916-924. doi: 10.1039/d2ay01549h. PMID: 36373982.
Saelens JW, Sweeney MI, Viswanathan G, Xet-Mull AM, Jurcic Smith KL, Sisk DM, Hu DD, Cronin RM, Hughes EJ, Brewer WJ, Coers J, Champion MM, Champion PA, Lowe CB, Smith CM, Lee S, Stout JE, Tobin DM. An ancestral mycobacterial effector promotes dissemination of infection. Cell. 2022 Nov 23;185(24):4507-4525.e18. doi: 10.1016/j.cell.2022.10.019. Epub 2022 Nov 9. PMID: 36356582; PMCID: PMC9691622.
Cronin RM, Ferrell MJ, Cahir CW, Champion MM, Champion PA. Proteo-genetic analysis reveals clear hierarchy of ESX-1 secretion in Mycobacterium marinum. Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2123100119. doi: 10.1073/pnas.2123100119. Epub 2022 Jun 7. PMID: 35671426; PMCID: PMC9214503.
Smith C, Canestrari JG, Wang AJ, Champion MM, Derbyshire KM, Gray TA, Wade JT. Pervasive translation in Mycobacterium tuberculosis. Elife. 2022 Mar 28;11:e73980. doi: 10.7554/eLife.73980. PMID: 35343439; PMCID: PMC9094748.
Huge BJ, Young K, Kerr C, Champion MM, Dovichi NJ. 3-D printed injection system for capillary electrophoresis. Anal Methods. 2022 Mar 17;14(11):1163-1168. doi: 10.1039/d2ay00075j. PMID: 35254370; PMCID: PMC8934206.
Ahrens CH, Wade JT, Champion MM, Langer JD. A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry. J Bacteriol. 2022 Jan 18;204(1):e0035321. doi: 10.1128/JB.00353-21. Epub 2021 Nov 8. PMID: 34748388; PMCID: PMC8765459.
Nicholson KR, Mousseau CB, Champion MM, Champion PA. The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens. PLoS Pathog. 2021 Jan 7;17(1):e1009124. doi: 10.1371/journal.ppat.1009124. PMID: 33411813; PMCID: PMC7790235.
Yan X, Sun L, Dovichi NJ, Champion MM. Minimal deuterium isotope effects in quantitation of dimethyl-labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry. Electrophoresis. 2020 Aug;41(15):1374-1378. doi: 10.1002/elps.202000051. Epub 2020 Jun 16. PMID: 32548848; PMCID: PMC7540333.
Canestrari JG, Lasek-Nesselquist E, Upadhyay A, Rofaeil M, Champion MM, Wade JT, Derbyshire KM, Gray TA. Polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene expression in mycobacteria. Mol Microbiol. 2020 Jul;114(1):93-108. doi: 10.1111/mmi.14498. Epub 2020 Apr 9. PMID: 32181921; PMCID: PMC8764745.
Sanchez KG, Ferrell MJ, Chirakos AE, Nicholson KR, Abramovitch RB, Champion MM, Champion PA. EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System. mBio. 2020 Feb 4;11(1):e02807-19. doi: 10.1128/mBio.02807-19. PMID: 32019792; PMCID: PMC7002343.
Bosserman RE, Nicholson KR, Champion MM, Champion PA. A New ESX-1 Substrate in Mycobacterium marinum That Is Required for Hemolysis but Not Host Cell Lysis. J Bacteriol. 2019 Jun 21;201(14):e00760-18. doi: 10.1128/JB.00760-18. PMID: 30833360; PMCID: PMC6597391.
Huge BJ, Champion MM, Dovichi NJ. Capillary Zone Electrophoresis with Fraction Collection for Separation, Culturing, and Identification of Bacteria from an Environmental Microbiome. Anal Chem. 2019 Apr 2;91(7):4649-4655. doi: 10.1021/acs.analchem.8b05984. Epub 2019 Mar 11. PMID: 30801180.
Thompson CR, Champion MM, Champion PA. Quantitative N-Terminal Footprinting of Pathogenic Mycobacteria Reveals Differential Protein Acetylation. J Proteome Res. 2018 Sep 7;17(9):3246-3258. doi: 10.1021/acs.jproteome.8b00373. Epub 2018 Aug 16. PMID: 30080413; PMCID: PMC6264890.
