Lundgren TJ, Clark PL, Champion MM. Fit for Purpose Approach To Evaluate Detection of Amino Acid Substitutions in Shotgun Proteomics. J Proteome Res. 2024 Mar 13. doi: 10.1021/acs.jproteome.3c00730. Epub ahead of print. PMID: 38478054.
Hu DD, Weaver SD, Collars OA, Champion PA, Champion MM. N-terminal proteomics of Mycobacterium marinum using bottom-up label-free quantitative analysis in data-dependent acquisition mode on a timsTOF Pro mass spectrometer. Microbiol Resour Announc. 2024 Mar 13:e0126323. doi: 10.1128/mra.01263-23. Epub ahead of print. PMID: 38477461.
Huge, B.J., Kerr, C.M., Wanigasinghe, S. Champion, M.M., & Dovichi, N.J. Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B. Sci Rep 13, 22269 (2023). https://doi.org/10.1038/s41598-023-49669-y
Collars, O. A., Jones, B. S., Hu, D. D., Weaver, S. D., Sherman, T. A., Champion, M. M., & Champion, P. A. (2023). An N-acetyltransferase required for ESAT-6 N-terminal acetylation and virulence in Mycobacterium marinum. MBio. https://doi.org/10.1128/mbio.00987-23
Weaver SD, DeRosa CM, Schultz SR, Champion MM. PrIntMap-R: An Online Application for Intraprotein Intensity and Peptide Visualization from Bottom-Up Proteomics. J Proteome Res. 2023 Feb 3;22(2):432-441. doi: 10.1021/acs.jproteome.2c00606. Epub 2023 Jan 18. PMID: 36652611.
Mousseau CB, Pierre CA, Hu DD, Champion MM. Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation. Anal Methods. 2023 Feb 16;15(7):916-924. doi: 10.1039/d2ay01549h. PMID: 36373982.
Saelens JW, Sweeney MI, Viswanathan G, Xet-Mull AM, Jurcic Smith KL, Sisk DM, Hu DD, Cronin RM, Hughes EJ, Brewer WJ, Coers J, Champion MM, Champion PA, Lowe CB, Smith CM, Lee S, Stout JE, Tobin DM. An ancestral mycobacterial effector promotes dissemination of infection. Cell. 2022 Nov 23;185(24):4507-4525.e18. doi: 10.1016/j.cell.2022.10.019. Epub 2022 Nov 9. PMID: 36356582; PMCID: PMC9691622.
Cronin RM, Ferrell MJ, Cahir CW, Champion MM, Champion PA. Proteo-genetic analysis reveals clear hierarchy of ESX-1 secretion in Mycobacterium marinum. Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2123100119. doi: 10.1073/pnas.2123100119. Epub 2022 Jun 7. PMID: 35671426; PMCID: PMC9214503.
Smith C, Canestrari JG, Wang AJ, Champion MM, Derbyshire KM, Gray TA, Wade JT. Pervasive translation in Mycobacterium tuberculosis. Elife. 2022 Mar 28;11:e73980. doi: 10.7554/eLife.73980. PMID: 35343439; PMCID: PMC9094748.
Huge BJ, Young K, Kerr C, Champion MM, Dovichi NJ. 3-D printed injection system for capillary electrophoresis. Anal Methods. 2022 Mar 17;14(11):1163-1168. doi: 10.1039/d2ay00075j. PMID: 35254370; PMCID: PMC8934206.
Ahrens CH, Wade JT, Champion MM, Langer JD. A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry. J Bacteriol. 2022 Jan 18;204(1):e0035321. doi: 10.1128/JB.00353-21. Epub 2021 Nov 8. PMID: 34748388; PMCID: PMC8765459.
Nicholson KR, Mousseau CB, Champion MM, Champion PA. The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens. PLoS Pathog. 2021 Jan 7;17(1):e1009124. doi: 10.1371/journal.ppat.1009124. PMID: 33411813; PMCID: PMC7790235.
Yan X, Sun L, Dovichi NJ, Champion MM. Minimal deuterium isotope effects in quantitation of dimethyl-labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry. Electrophoresis. 2020 Aug;41(15):1374-1378. doi: 10.1002/elps.202000051. Epub 2020 Jun 16. PMID: 32548848; PMCID: PMC7540333.
Canestrari JG, Lasek-Nesselquist E, Upadhyay A, Rofaeil M, Champion MM, Wade JT, Derbyshire KM, Gray TA. Polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene expression in mycobacteria. Mol Microbiol. 2020 Jul;114(1):93-108. doi: 10.1111/mmi.14498. Epub 2020 Apr 9. PMID: 32181921; PMCID: PMC8764745.
Sanchez KG, Ferrell MJ, Chirakos AE, Nicholson KR, Abramovitch RB, Champion MM, Champion PA. EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System. mBio. 2020 Feb 4;11(1):e02807-19. doi: 10.1128/mBio.02807-19. PMID: 32019792; PMCID: PMC7002343.
Bosserman RE, Nicholson KR, Champion MM, Champion PA. A New ESX-1 Substrate in Mycobacterium marinum That Is Required for Hemolysis but Not Host Cell Lysis. J Bacteriol. 2019 Jun 21;201(14):e00760-18. doi: 10.1128/JB.00760-18. PMID: 30833360; PMCID: PMC6597391.
Huge BJ, Champion MM, Dovichi NJ. Capillary Zone Electrophoresis with Fraction Collection for Separation, Culturing, and Identification of Bacteria from an Environmental Microbiome. Anal Chem. 2019 Apr 2;91(7):4649-4655. doi: 10.1021/acs.analchem.8b05984. Epub 2019 Mar 11. PMID: 30801180.
Thompson CR, Champion MM, Champion PA. Quantitative N-Terminal Footprinting of Pathogenic Mycobacteria Reveals Differential Protein Acetylation. J Proteome Res. 2018 Sep 7;17(9):3246-3258. doi: 10.1021/acs.jproteome.8b00373. Epub 2018 Aug 16. PMID: 30080413; PMCID: PMC6264890.
Hu DD, Weaver SD, Collars OA, Champion PA, Champion MM. N-terminal proteomics of Mycobacterium marinum using bottom-up label-free quantitative analysis in data-dependent acquisition mode on a timsTOF Pro mass spectrometer. Microbiol Resour Announc. 2024 Mar 13:e0126323. doi: 10.1128/mra.01263-23. Epub ahead of print. PMID: 38477461.
Huge, B.J., Kerr, C.M., Wanigasinghe, S. Champion, M.M., & Dovichi, N.J. Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B. Sci Rep 13, 22269 (2023). https://doi.org/10.1038/s41598-023-49669-y
Collars, O. A., Jones, B. S., Hu, D. D., Weaver, S. D., Sherman, T. A., Champion, M. M., & Champion, P. A. (2023). An N-acetyltransferase required for ESAT-6 N-terminal acetylation and virulence in Mycobacterium marinum. MBio. https://doi.org/10.1128/mbio.00987-23
Weaver SD, DeRosa CM, Schultz SR, Champion MM. PrIntMap-R: An Online Application for Intraprotein Intensity and Peptide Visualization from Bottom-Up Proteomics. J Proteome Res. 2023 Feb 3;22(2):432-441. doi: 10.1021/acs.jproteome.2c00606. Epub 2023 Jan 18. PMID: 36652611.
Mousseau CB, Pierre CA, Hu DD, Champion MM. Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation. Anal Methods. 2023 Feb 16;15(7):916-924. doi: 10.1039/d2ay01549h. PMID: 36373982.
Saelens JW, Sweeney MI, Viswanathan G, Xet-Mull AM, Jurcic Smith KL, Sisk DM, Hu DD, Cronin RM, Hughes EJ, Brewer WJ, Coers J, Champion MM, Champion PA, Lowe CB, Smith CM, Lee S, Stout JE, Tobin DM. An ancestral mycobacterial effector promotes dissemination of infection. Cell. 2022 Nov 23;185(24):4507-4525.e18. doi: 10.1016/j.cell.2022.10.019. Epub 2022 Nov 9. PMID: 36356582; PMCID: PMC9691622.
Cronin RM, Ferrell MJ, Cahir CW, Champion MM, Champion PA. Proteo-genetic analysis reveals clear hierarchy of ESX-1 secretion in Mycobacterium marinum. Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2123100119. doi: 10.1073/pnas.2123100119. Epub 2022 Jun 7. PMID: 35671426; PMCID: PMC9214503.
Smith C, Canestrari JG, Wang AJ, Champion MM, Derbyshire KM, Gray TA, Wade JT. Pervasive translation in Mycobacterium tuberculosis. Elife. 2022 Mar 28;11:e73980. doi: 10.7554/eLife.73980. PMID: 35343439; PMCID: PMC9094748.
Huge BJ, Young K, Kerr C, Champion MM, Dovichi NJ. 3-D printed injection system for capillary electrophoresis. Anal Methods. 2022 Mar 17;14(11):1163-1168. doi: 10.1039/d2ay00075j. PMID: 35254370; PMCID: PMC8934206.
Ahrens CH, Wade JT, Champion MM, Langer JD. A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry. J Bacteriol. 2022 Jan 18;204(1):e0035321. doi: 10.1128/JB.00353-21. Epub 2021 Nov 8. PMID: 34748388; PMCID: PMC8765459.
Nicholson KR, Mousseau CB, Champion MM, Champion PA. The genetic proteome: Using genetics to inform the proteome of mycobacterial pathogens. PLoS Pathog. 2021 Jan 7;17(1):e1009124. doi: 10.1371/journal.ppat.1009124. PMID: 33411813; PMCID: PMC7790235.
Yan X, Sun L, Dovichi NJ, Champion MM. Minimal deuterium isotope effects in quantitation of dimethyl-labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry. Electrophoresis. 2020 Aug;41(15):1374-1378. doi: 10.1002/elps.202000051. Epub 2020 Jun 16. PMID: 32548848; PMCID: PMC7540333.
Canestrari JG, Lasek-Nesselquist E, Upadhyay A, Rofaeil M, Champion MM, Wade JT, Derbyshire KM, Gray TA. Polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene expression in mycobacteria. Mol Microbiol. 2020 Jul;114(1):93-108. doi: 10.1111/mmi.14498. Epub 2020 Apr 9. PMID: 32181921; PMCID: PMC8764745.
Sanchez KG, Ferrell MJ, Chirakos AE, Nicholson KR, Abramovitch RB, Champion MM, Champion PA. EspM Is a Conserved Transcription Factor That Regulates Gene Expression in Response to the ESX-1 System. mBio. 2020 Feb 4;11(1):e02807-19. doi: 10.1128/mBio.02807-19. PMID: 32019792; PMCID: PMC7002343.
Bosserman RE, Nicholson KR, Champion MM, Champion PA. A New ESX-1 Substrate in Mycobacterium marinum That Is Required for Hemolysis but Not Host Cell Lysis. J Bacteriol. 2019 Jun 21;201(14):e00760-18. doi: 10.1128/JB.00760-18. PMID: 30833360; PMCID: PMC6597391.
Huge BJ, Champion MM, Dovichi NJ. Capillary Zone Electrophoresis with Fraction Collection for Separation, Culturing, and Identification of Bacteria from an Environmental Microbiome. Anal Chem. 2019 Apr 2;91(7):4649-4655. doi: 10.1021/acs.analchem.8b05984. Epub 2019 Mar 11. PMID: 30801180.
Thompson CR, Champion MM, Champion PA. Quantitative N-Terminal Footprinting of Pathogenic Mycobacteria Reveals Differential Protein Acetylation. J Proteome Res. 2018 Sep 7;17(9):3246-3258. doi: 10.1021/acs.jproteome.8b00373. Epub 2018 Aug 16. PMID: 30080413; PMCID: PMC6264890.